Reporter

Part:BBa_K422014:Design

Designed by: Katharina Zwicky   Group: iGEM10_ETHZ_Basel   (2010-10-25)

mCyPet (AarI A-part)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 733
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 733
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 733
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 733
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. mCyPet-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Source

Codon optimized.

References

http://2010.igem.org/Team:ETHZ_Basel

Ohashi, Galiacy, Briscoe and Erickson: An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein Science. 2007; 16.

Nguyen and Daugherty: Evolutionary optimization of fluorescent proteins for intracellular FRET. Nature Biotechnology. 2005; 23.